1QDW
N-TERMINAL DOMAIN, VOLTAGE-GATED POTASSIUM CHANNEL KV1.2 RESIDUES 33-119
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.260, 78.740, 125.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.226 * |
| Rwork | 0.223 |
| R-free | 0.26500 * |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.130 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 * | 0.283 * |
| Number of reflections | 42293 | |
| <I/σ(I)> | 10.9 | |
| Completeness [%] | 94.5 * | 83.7 |
| Redundancy | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | 18 * | 9% PEG 1500, 5% N-PROPANOL, 120 MM GDHCL 50 MM MES PH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG1500 | 9 (%) | |
| 3 | 1 | reservoir | n-propanol | 5 (%) | |
| 4 | 1 | reservoir | Gdm HCl | 120 (mM) | |
| 5 | 1 | reservoir | MES | 50 (mM) | |
| 6 | 1 | reservoir | water | 0-15 (%) |
