1QBA

BACTERIAL CHITOBIASE, GLYCOSYL HYDROLASE FAMILY 20

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, Hamburg
Beamline	X11
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-05-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	110.700, 99.900, 87.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 * - 1.900 *
R-factor	0.146
Rwork	0.139
R-free	0.19600
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	0.026
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *	1.880
High resolution limit [Å]	1.900 *	1.850
Rmerge	0.062	0.276
Number of reflections	83596 *
<I/σ(I)>	15.8	3.2
Completeness [%]	99.9	99.8 *
Redundancy	5.5	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.6		CRYSTAL WERE GROWN AT ROOM TEMPERATURE FROM HANGING DROPS CONTAINING 62% SATURATED AMMONIUM SULFATE, 1.5% ISOPROPANOL AND 100 MM CACODYLATE, PH 5.6., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium salfate	61.5 (%)
2	1	1	isopropanol	1.5 (%)
3	1	1	cacodylate	100 (mM)