1QA6
CRYSTAL STRUCTURE OF A CONSERVED RIBOSOMAL PROTEIN-RNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-09 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 150.675, 150.675, 63.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.32100 |
RMSD bond length | 0.020 |
RMSD bond angle | 4.118 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.930 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.055 | 0.240 * |
Total number of observations | 89078 * | |
Number of reflections | 17542 | |
<I/σ(I)> | 23.3 | |
Completeness [%] | 94.5 | 88.7 |
Redundancy | 5.0 * | 4.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 37 * | PEG 600, MAGNESIUM ACETATE, COBALT HEXAMINE CHLORIDE, SODIUM CACODYLATE, KCL, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 310.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MAGNESIUM ACETATE | ||
2 | 1 | 1 | [CO(NH3)6]CL3 | ||
3 | 1 | 1 | KCL | ||
4 | 1 | 1 | CACODYLATE | ||
5 | 1 | 1 | PEG 600 | ||
6 | 1 | 2 | PEG 600 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | sodium cacodylate | 50 (mM) | |
2 | 1 | reservoir | PEG600 | 15 (%) | |
3 | 1 | reservoir | 80 (mM) | ||
4 | 1 | reservoir | 100 (mM) | ||
5 | 1 | reservoir | 0.2 (mM) |