1Q99
Crystal structure of the Saccharomyces cerevisiae SR protein kinsae, Sky1p, complexed with the non-hydrolyzable ATP analogue, AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2001-11-15 |
| Detector | SBC-2 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.413, 88.947, 134.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.950 - 2.100 * |
| Rwork | 0.220 |
| R-free | 0.26010 * |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.270 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SORTRF |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.110 | 2.180 |
| High resolution limit [Å] | 2.100 * | 2.110 |
| Rmerge | 0.085 * | 46.700 * |
| Total number of observations | 280589 * | |
| Number of reflections | 49644 * | |
| Completeness [%] | 96.5 * | 86.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | Nolen, B., (2001) Nat.Struct.Biol., 8, 176. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | ATP | 0.0066 (mM) | |
| 3 | 1 | drop | 0.0017 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 5 | 1 | reservoir | ethylene glycol | 15 (%(v/v)) | |
| 6 | 1 | reservoir | sodium acetate | 10 (mM) |
