1Q5I
Crystal structure of bacteriorhodopsin mutant P186A crystallized from bicelles
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.629, 108.721, 56.093 |
| Unit cell angles | 90.00, 113.46, 90.00 |
Refinement procedure
| Resolution | 500.000 - 2.300 |
| R-factor | 0.198 |
| Rwork | 0.194 |
| R-free | 0.25500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1py6 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.116 | |
| Number of reflections | 24112 | |
| <I/σ(I)> | 23 | |
| Completeness [%] | 96.6 | 92.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 3.7 * | 310 | DMPC, CHAPSO, sodium phosphate, hexanediol, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 310.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2 (M) | pH4.5 | |
| 2 | 1 | reservoir | 0.8 (M) | pH3.7 | |
| 3 | 1 | reservoir | hexanediol | 0.09 (M) |
