1Q31
Crystal Structure of the Tobacco Etch Virus Protease C151A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-13 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.933 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.587, 41.544, 96.063 |
Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
Resolution | 36.580 - 2.700 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.30200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1lvb |
RMSD bond length | 0.011 |
RMSD bond angle | 1.720 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 13436 | |
Completeness [%] | 88.0 | 93.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | lithium sulphate monohydrate, Tris HCL, PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |