1PZG
T.gondii LDH1 complexed with APAD and sulfate at 1.6 Angstroms
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-12-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.935, 124.978, 86.388 |
Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.600 |
R-factor | 0.176 |
Rwork | 0.176 |
R-free | 0.19760 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pzf |
RMSD bond length | 0.010 |
RMSD bond angle | 1.470 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASING (FROM ISOMORPHOUS STRUCTURE) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.036 | 0.346 |
Total number of observations | 451492 * | |
Number of reflections | 167180 | |
<I/σ(I)> | 25.3 | 2.6 |
Completeness [%] | 91.8 | 92.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.5 | 293 | 0.9 M ammonium sulfate, 100 mM acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | drop | cosubstrate | 10 (mM) | |
3 | 1 | drop | oxalate | 5 (mM) | or oxamate |
4 | 1 | reservoir | ammonium sulfate | 0.85-0.90 (M) | |
5 | 1 | reservoir | acetate | 100 (mM) | pH4.5 |