1PNR
PURINE REPRESSOR-HYPOXANTHINE-PURF-OPERATOR COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Detector technology | AREA DETECTOR |
Detector | SDMS |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 175.850, 94.790, 81.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.700 |
R-factor | 0.171 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.400 |
Refinement software | TNT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.700 * |
Rmerge | 0.056 * |
Number of reflections | 18242 * |
Completeness [%] | 97.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 293 | Schumacher, M.A., (1994) J.Mol.Biol, 242, 302. * |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | WATER | ||
11 | 1 | 2 | PEG 4000 | ||
12 | 1 | 2 | NH4 SULFATE | ||
13 | 1 | 2 | [CO(NH3)6]3+ | ||
14 | 1 | 2 | NH4 PHOSPHATE | ||
2 | 1 | 1 | HYPOXANTHINE | ||
3 | 1 | 1 | DTT | ||
4 | 1 | 1 | PHOSPHATE | ||
5 | 1 | 1 | NA CACODYLATE | ||
6 | 1 | 1 | PEG 4000 | ||
7 | 1 | 1 | NH4 SULFATE | ||
8 | 1 | 1 | [CO(NH3)6]3+ | ||
9 | 1 | 1 | NH4 PHOSPHATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5 (mM) | |
10 | 1 | reservoir | PEG4000 | 25 (%) | |
11 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | pH7.5 |
2 | 1 | drop | DTT | 1.0 (mM) | |
3 | 1 | drop | sodium potassium phosphate | 160 (mM) | pH7.4 |
4 | 1 | drop | DNA | 0.6 (mM) | |
5 | 1 | drop | sodium cacodylate | 50 (mM) | pH6.9 |
6 | 1 | drop | PEG4000 | 25 (%) | |
7 | 1 | drop | ammonium sulfate | 0.4 (M) | |
8 | 1 | drop | cobalt hexammine | 50 (mM) | |
9 | 1 | drop | ammonium phosphate | 0.1 (M) | pH7.5 |