1PLG
EVIDENCE FOR THE EXTENDED HELICAL NATURE OF POLYSACCHARIDE EPITOPES. THE 2.8 ANGSTROMS RESOLUTION STRUCTURE AND THERMODYNAMICS OF LIGAND BINDING OF AN ANTIGEN BINDING FRAGMENT SPECIFIC FOR ALPHA-(2->8)-POLYSIALIC ACID
Experimental procedure
Detector technology | AREA DETECTOR |
Collection date | 1991-04-24 |
Detector | SDMS |
Wavelength(s) | 1.5418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.140, 91.210, 141.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.800 |
R-factor | 0.164 |
Rwork | 0.164 |
RMSD bond length | 0.014 |
RMSD bond angle | 0.052 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.800 * |
Rmerge | 0.054 |
Total number of observations | 87625 * |
Number of reflections | 10590 * |
Completeness [%] | 99.8 * |
Redundancy | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab | 6.7 (mg/ml) | |
2 | 1 | drop | reservoir solution | 0.006 ml | |
3 | 1 | reservoir | PEG4000 | 16-18 (%) | |
4 | 1 | reservoir | HEPES | 0.1 (M) | |
5 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
6 | 1 | reservoir | 0.01 (M) |