1PK0
Crystal Structure of the EF3-CaM complexed with PMEApp
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2003-04-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 116.263, 165.759, 342.409 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.980 - 3.300 |
Rwork | 0.264 |
R-free | 0.30200 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | ef3-cam |
RMSD bond length | 0.011 |
RMSD bond angle | 22.100 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.081 * | 0.390 |
Number of reflections | 49594 | |
<I/σ(I)> | 7.4 | 5.35 |
Completeness [%] | 99.4 * | 99.8 |
Redundancy | 7.3 * | 6.7 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 273 | Yan, S.Z., (2003) J.Biol.Chem., 278, 25990. * |