1PJM
Mouse Importin alpha-bipartite NLS from human retinoblastoma protein Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-08-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.000, 89.179, 100.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.500 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | : 1ial |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.073 | 0.403 |
| Total number of observations | 249870 * | |
| Number of reflections | 25382 | |
| <I/σ(I)> | 19 | 2.8 |
| Completeness [%] | 96.0 | 98.3 |
| Redundancy | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 293 | Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.7 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.6 (M) | pH6.0 |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) |
