1PG7
Murine 6A6 Fab in complex with humanized anti-Tissue Factor D3H44 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-21 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.917 |
Spacegroup name | P 1 |
Unit cell lengths | 78.890, 85.780, 92.370 |
Unit cell angles | 77.48, 75.92, 63.33 |
Refinement procedure
Resolution | 50.000 - 2.500 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1JPS 1gig |
RMSD bond length | 0.009 |
RMSD bond angle | 28.100 * |
Data reduction software | HKL-2000 |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | AMoRE |
Refinement software | X-PLOR (98.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.129 | 0.477 |
Total number of observations | 310288 * | |
Number of reflections | 72248 | 7157 * |
<I/σ(I)> | 12 | |
Completeness [%] | 99.0 | 98 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 18 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | pH8.0 |
3 | 1 | reservoir | PEG3350 | 20 (%) | |
4 | 1 | reservoir | Tris | 100 (mM) | pH7.0 |