1P7S
T4 LYSOZYME CORE REPACKING MUTANT V103I/TA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.23979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.787, 54.862, 88.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.600 - 1.500 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.086 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 0.351 |
| Number of reflections | 23577 | |
| <I/σ(I)> | 7.4 | 2.2 |
| Completeness [%] | 95.3 | 78 * |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 277 | 2M K/Na phosphate, NaCl, BME, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 2 | 1 | reservoir | PEG3400 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | isopropanol | 5 (%(v/v)) |
