1P1Q
Crystal structure of the GluR2 ligand binding core (S1S2J) L650T mutant in complex with AMPA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2001-03-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 113.942, 163.642, 47.116 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.222 |
| Rwork | 0.217 |
| R-free | 0.27000 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.310 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.070 |
| Number of reflections | 55769 | |
| <I/σ(I)> | 25.3 | |
| Completeness [%] | 91.9 | 64.9 |
| Redundancy | 3.98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 4 * | PEG8000, cacodylate, zinc acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 12-16 (%) | |
| 2 | 1 | reservoir | zinc acetate | 0.1-0.3 (M) | |
| 3 | 1 | reservoir | cacodylate | 0.1 (M) | pH6.5 |
