1OZL
Crystal Structures of the Ferric, Ferrous, and Ferrous-NO Forms of the Asp140Ala Mutant of Human Heme Oxygenase-1: Catalytic Implications
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 119 |
| Detector technology | CCD |
| Collection date | 2002-10-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.435, 54.415, 71.004 |
| Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.580 |
| Rwork | 0.205 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.038 * | 0.401 * |
| Total number of observations | 208425 * | |
| Number of reflections | 63001 * | |
| <I/σ(I)> | 18.2 | 2.14 |
| Completeness [%] | 99.1 * | 94.2 |
| Redundancy | 9.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 * | 301 | ammomium sulphate, 1-6 hexanediol, HEPES, water, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 301K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.08 (M) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | 1,6-hexanediol | 0.9 (%(v/v)) | |
| 4 | 1 | drop | protein | 45 (mg/ml) | |
| 5 | 1 | drop | potassium phosphate | 20 (mM) | pH7.4 |
