1ONL
Crystal structure of Thermus thermophilus HB8 H-protein of the glycine cleavage system
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-22 |
Detector | MARRESEARCH |
Wavelength(s) | 1.00 |
Spacegroup name | P 65 |
Unit cell lengths | 55.775, 55.775, 191.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 * - 2.500 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.25600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hpc |
RMSD bond length | 0.006 |
RMSD bond angle | 24.400 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 * | 2.580 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.109 | 0.355 |
Total number of observations | 135290 * | |
Number of reflections | 11730 | |
<I/σ(I)> | 13.7 | 2.5 |
Completeness [%] | 99.6 * | 98.5 |
Redundancy | 11.5 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 291 | ammonium phosphate, glycerol, pH 4.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
3 | 1 | drop | 150 (mM) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | ammonium phosphate | 0.4 (M) | |
6 | 1 | reservoir | glycerol | 24 (%(v/v)) | pH4.3 |