1OJA
HUMAN MONOAMINE OXIDASE B IN COMPLEX WITH ISATIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 130.340, 221.964, 85.966 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.18209 |
Rwork | 0.181 |
R-free | 0.20400 * |
Structure solution method | OTHER |
RMSD bond length | 0.012 * |
RMSD bond angle | 1.400 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.083 | 0.339 |
Number of reflections | 128815 | |
<I/σ(I)> | 6.4 | 2.1 |
Completeness [%] | 94.4 | 76.7 |
Redundancy | 4.3 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 * | 4 * | pH 6.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 12 (%(w/v)) | |
2 | 1 | reservoir | lithium sulfate | 70 (mM) | |
3 | 1 | reservoir | ADA | 100 (mM) | pH6.5 |
4 | 1 | reservoir | protein | 2 (mg/ml) | |
5 | 1 | reservoir | Zwittergent3-12 | 8.5 (mM) | |
6 | 1 | reservoir | potassium phosphate | 25 (mM) | pH7.5 |