1OAS

O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1997-04-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	54.600, 98.200, 145.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.172
R-free	0.20000
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	23.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.150
Rmerge	0.064
Total number of observations	155500 *
Number of reflections	43785
Completeness [%]	96.8
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	Tris-HCl	0.1 (M)
4	1	reservoir		0.2 (M)