1O9R
The X-ray crystal structure of Agrobacterium tumefaciens Dps, a member of the family that protect DNA without binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 106.046, 90.349, 105.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.450 |
| R-factor | 0.18 |
| Rwork | 0.170 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dps |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.450 |
| Rmerge | 0.053 |
| Number of reflections | 179135 |
| Completeness [%] | 96.0 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 293 * | HEPES 0.1 M, PH 7.0-7.8, ETHYLENE GLYCOL IN A RANGE BETWEEN 16%-24% V/V |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 30 (mM) | pH7.5 |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | protein | 7 (mg/ml) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) | pH7.0-7.8 |
| 5 | 1 | reservoir | ethylene glycol | 16-24 (%(v/v)) |
