1O6U
The Crystal Structure of Human Supernatant Protein Factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.794, 84.240, 87.111 |
| Unit cell angles | 116.26, 102.39, 99.87 |
Refinement procedure
| Resolution | 20.000 * - 2.050 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.22700 * |
| Structure solution method | OTHER |
| RMSD bond length | 0.005 |
| RMSD bond angle | 22.500 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 2.050 | 2.000 |
| Rmerge | 0.093 | 0.251 |
| Total number of observations | 715326 * | |
| Number of reflections | 184572 | |
| <I/σ(I)> | 15.7 | 3.9 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.4 * | 18 * | pH 8.30 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 20 (%(w/v)) | |
| 2 | 1 | reservoir | glycerol | 5 (%(w/v)) | |
| 3 | 1 | reservoir | sodium citrate | 200 (mM) | pH8.3 |
| 4 | 1 | drop | EDTA | 2 (mM) | |
| 5 | 1 | drop | dithiothreitol | 2 (mM) | |
| 6 | 1 | drop | 40 (mM) | ||
| 7 | 1 | drop | Tris-HCl | 20 (mM) | pH7.4 |
| 8 | 1 | drop | protein | 20 (mg/ml) |
