1O2E
Structure of the triple mutant (K53,56,120M) + Anisic acid complex of phospholipase A2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | OTHER |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-28 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 46.610, 46.610, 102.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.800 - 2.600 |
Rwork | 0.187 |
R-free | 0.24100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.800 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.151 | 0.263 |
Number of reflections | 3957 | |
Completeness [%] | 92.0 | 94.9 |
Redundancy | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | vapor diffusion method | 7.2 | 293 | 50 mM Tris Buffer, MPD,70% MPD reservoir,17-20Mg/ml prot,5 mM CaCl2, 1 microlitre of anisic acid, pH 7.2, vapor diffusion method, temperature 293.0K |