1NWR
Crystal structure of human cartilage gp39 (HC-gp39)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 127.300, 127.300, 107.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 2.600* |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.24000 * |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.060 * | 0.259 |
| Total number of observations | 205355 * | |
| Number of reflections | 52490 | |
| <I/σ(I)> | 18.2 | 4.1 |
| Completeness [%] | 99.6 * | 96.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 277 | PEG8000, NaCl, Na-citrate buffer, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.10 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 1 (M) | ||
| 3 | 1 | drop | BES | 10 (mM) | pH7.2 |
| 4 | 1 | reservoir | PEG8000 | 10 (%) | |
| 5 | 1 | reservoir | 0.5 (M) | ||
| 6 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.1 |
