1NWM
GAT domain of human GGA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2002-12-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9199 |
| Spacegroup name | H 3 |
| Unit cell lengths | 76.660, 76.660, 60.816 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.100 * - 2.400 |
| R-factor | 0.25369 |
| Rwork | 0.252 |
| R-free | 0.28600 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.330 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.296 |
| Number of reflections | 5195 | 474 * |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 98.7 * | 90.1 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 25 * | polyethylene glycol 4000, iso-propanol, sodium citrate, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | pH5.6 |
| 3 | 1 | reservoir | PEG4000 | 20 (%) | |
| 4 | 1 | reservoir | isopropyl alcohol | 20 (%) |
