1NWK
CRYSTAL STRUCTURE OF MONOMERIC ACTIN IN THE ATP STATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9799 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.216, 37.368, 85.027 |
Unit cell angles | 90.00, 108.13, 90.00 |
Refinement procedure
Resolution | 24.400 * - 1.850 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | IJ6Z |
RMSD bond length | 0.006 |
RMSD bond angle | 22.200 * |
Data reduction software | DENZO (1.97.2) |
Data scaling software | SCALEPACK (1.97.2) |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.400 | 1.970 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.091 | 0.290 |
Number of reflections | 28512 | |
<I/σ(I)> | 12.1312 | 7.2 |
Completeness [%] | 98.2 | 90.2 |
Redundancy | 7.5 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 20 * | PEG 3350, CaCl2, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 393K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 9-12 (mg/ml) | |
2 | 1 | reservoir | PEG3350 | 18 (%) | |
3 | 1 | reservoir | ACES | 9 (mM) | pH7.0 |
4 | 1 | reservoir | 365 (mM) | ||
5 | 1 | reservoir | dimethyl sulfoxide | 9 (%) |