1NTF
Crystal Structure of Cimex Nitrophorin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-07-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.200, 42.100, 65.850 |
| Unit cell angles | 90.00, 95.88, 90.00 |
Refinement procedure
| Resolution | 21.000 - 1.800 |
| R-factor | 0.15323 |
| Rwork | 0.151 |
| R-free | 0.20000 * |
| Structure solution method | MAD |
| RMSD bond length | 0.022 * |
| RMSD bond angle | 2.000 * |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 * | 0.310 * |
| Number of reflections | 24988 | |
| <I/σ(I)> | 11 | 2.4 |
| Completeness [%] | 98.5 | 96.5 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | PEG 4000, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH8.6 |
| 4 | 1 | drop | sodium citrate | 20 (mM) | pH5.6, or 100mMpotassium phosphate pH8.1 |
