1NQL
Structure of the extracellular domain of human epidermal growth factor (EGF) receptor in an inactive (low pH) complex with EGF.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-09-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.94 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.170, 103.660, 101.490 |
| Unit cell angles | 90.00, 119.27, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.24747 |
| Rwork | 0.241 |
| R-free | 0.31000 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m6b |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 1.890 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.048 * | 0.345 * |
| Total number of observations | 90194 * | |
| Number of reflections | 26060 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 98.0 | 95.4 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 297 | PEG3400,ammonium sulfate, magnesium sulfate, sodium citrate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 25 (mM) | pH8.0 |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | reservoir | PEG3400 | 12 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 5 | 1 | reservoir | 50 (mM) | ||
| 6 | 1 | reservoir | sodium citrate | 50 (mM) | pH5.0 |
