1NPF
MYOGLOBIN (HORSE HEART) WILD-TYPE COMPLEXED WITH NITRIC OXIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-11-06 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.437, 28.728, 62.976 |
Unit cell angles | 90.00, 105.81, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.900 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.25000 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dwr |
RMSD bond length | 0.009 * |
RMSD bond angle | 1.055 * |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.052 | 0.159 |
Total number of observations | 34504 * | |
Number of reflections | 17516 | 1687 * |
<I/σ(I)> | 11.3 | 3.4 |
Completeness [%] | 93.1 | 91.1 |
Redundancy | 1.95 | 1.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | Chu, K., (2000) Nature, 403, 921. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | ammonium sulfate | 1.7-1.8 (M) | |
3 | 1 | drop | Tris-HCl | 0.1 (M) | |
4 | 1 | reservoir | ammonium sulfate | 3.4-3.6 (M) | |
5 | 1 | reservoir | Tris-HCl | 0.1 (M) |