1NOK

COMPLEX OF GLYCOGEN PHOSPHORYLASE WITH A TRANSITION STATE ANALOGUE NOJIRIMYCIN TETRAZOLE AND PHOSPHATE IN THE T STATE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993
Detector	SIEMENS
Spacegroup name	P 43 21 2
Unit cell lengths	128.500, 128.500, 116.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.193
Rwork	0.193
RMSD bond length	0.017
RMSD bond angle	3.200
Data reduction software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.068
Total number of observations	82739 *
Number of reflections	37024 *
Completeness [%]	95.0 *	81 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	GPb	20-40 (mg/ml)
2	1	1	IMP	1 (mM)
3	1	1	spermine	1 (mM)
4	1	1	BES	10 (mM)
5	1	1	EDTA	0.1 (mM)
6	1	1	sodium azide	0.02 (%)