1NLV
Crystal Structure Of Dictyostelium Discoideum Actin Complexed With Ca ATP And Human Gelsolin Segment 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 1999-04-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.07018 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.446, 69.089, 56.570 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 23.000 - 1.800 |
Rwork | 0.210 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dag |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.338 | |
Number of reflections | 61207 | |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 4.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | lithium chloride, magnesium chloride, HEPES, ATP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |