1N9Z
INTEGRIN ALPHA M I DOMAIN MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 43 |
| Unit cell lengths | 44.220, 44.220, 99.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.247 |
| Rwork | 0.242 |
| R-free | 0.27700 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ido |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.710 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.111 * | 0.347 * |
| Total number of observations | 14631 * | |
| Number of reflections | 6234 * | |
| Completeness [%] | 95.5 | 97.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 23 * | PEG 3000, sodium chloride, hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 100 (mM) | ||
| 2 | 1 | drop | 5 (mM) | ||
| 3 | 1 | drop | Tris | 20 (mM) | pH8.0 |
| 4 | 1 | drop | protein | 10 (mg/ml) | |
| 5 | 1 | reservoir | 0.2 (M) | ||
| 6 | 1 | reservoir | PEG3000 | 20 (%) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
