1N8E
Fragment Double-D from Human Fibrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.000, 125.350, 312.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 4.500 |
| R-factor | 0.415 |
| Rwork | 0.415 |
| R-free | 0.41600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | fragment D of 1FZC |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 4.600 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.138 | |
| Total number of observations | 71273 * | |
| Number of reflections | 10214 | |
| Completeness [%] | 83.4 | 53.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 295 | PEG-3350, 50 mM Tris, 7.5 mM CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.0 |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | PEG3350 | 13.25 (%) | |
| 5 | 1 | reservoir | 10 (mM) | ||
| 6 | 1 | reservoir | Tris | 50 (mM) | pH8.0 |
