1N6J
Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 101 |
Detector technology | CCD |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 70.140, 70.140, 151.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 * - 2.200 |
R-factor | 0.243 |
Rwork | 0.243 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1egw |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.090 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.063 * | 0.319 * |
Total number of observations | 364592 * | |
Number of reflections | 18733 | |
<I/σ(I)> | 38 | 4.4 |
Completeness [%] | 92.7 * | 77.7 * |
Redundancy | 19.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 17 * | PEG 3500, sodium cholride, BTP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 3500 | ||
2 | 1 | 1 | sodium cholride | ||
3 | 1 | 1 | BTP | ||
4 | 1 | 1 | H2O | ||
5 | 1 | 2 | PEG 3500 | ||
6 | 1 | 2 | sodium cholride | ||
7 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | bis-Tris-propane | 50 (mM) | pH6.35-6.68 |
2 | 1 | reservoir | PEG1000 | 15 (%) | |
3 | 1 | reservoir | 50 (mM) | ||
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | 5 (mM) | ||
6 | 1 | reservoir | glycerol | 10 (%) | |
7 | 1 | reservoir | dithiothreitol | 5 (mM) | |
8 | 1 | reservoir | spermine | 2 (mM) |