1N4X
Structure of scFv 1696 at acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-01-06 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.930, 61.210, 57.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.340 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | |
| High resolution limit [Å] | 1.700 * | |
| Rmerge | 0.090 * | 0.360 * |
| Number of reflections | 45422 * | 5930 * |
| Completeness [%] | 91.0 * | 84.2 * |
| Redundancy | 4.89 * | 3.34 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.3 * | 291 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium citrate | 0.1 (M) | pH3.5 |
| 2 | 1 | reservoir | PEG4000 | 17.5 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.175 (M) | |
| 4 | 1 | drop | protein | 5 (mg/ml) | |
| 5 | 1 | drop | Tris-HCl | 10 (mM) | pH7.3 |
