1N43
Streptavidin Mutant N23A with biotin at 1.89A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-19 |
Detector | RIGAKU RAXIS II |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.180, 97.200, 51.580 |
Unit cell angles | 90.00, 112.60, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 * |
R-factor | 0.1917 |
Rwork | 0.192 |
R-free | 0.30100 * |
Structure solution method | isomorphous replacement |
Starting model (for MR) | 1swe |
RMSD bond length | 0.005 |
RMSD bond angle | 0.021 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.900 * | |
Rmerge | 0.070 | 0.250 * |
Number of reflections | 27213 * | |
Completeness [%] | 71.0 | 43 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | 293 | MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20-30 (mg/ml) | |
2 | 1 | reservoir | MPD | 50 (%) |