1MVU
SINGLE CHAIN FV OF C219 HEAVY CHAIN V101L MUTANT IN COMPLEX WITH SYNTHETIC EPITOPE PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-06-10 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.733, 50.967, 58.636 |
| Unit cell angles | 90.00, 93.89, 90.00 |
Refinement procedure
| Resolution | 19.270 - 1.780 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.840 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Number of reflections | 23268 | |
| Completeness [%] | 98.3 | 96.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | PEG8000, lithium sulfate, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
