1MR7
Crystal Structure of Streptogramin A Acetyltransferase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-11-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97947, 0.97939, 0.96863 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 181.393, 183.001, 184.124 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.800 |
R-factor | 0.17792 |
Rwork | 0.176 |
R-free | 0.20700 * |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.240 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.438 |
Total number of observations | 1293725 * | |
Number of reflections | 140296 | |
<I/σ(I)> | 21.3 | 3.27 |
Completeness [%] | 97.2 * | 91.3 * |
Redundancy | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 290 | Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 290 | Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 290 | Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | protein | 0.15-0.25 (mM) | |
2 | 1 | reservoir | Tris-HCl | 1 (mM) | pH7.5 |