1MP9
TBP from a mesothermophilic archaeon, Sulfolobus acidocaldarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL24XU |
Synchrotron site | SPring-8 |
Beamline | BL24XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-21 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 0.834 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.900, 80.400, 86.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 25.000 * |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | |
Number of reflections | 31120 | |
Completeness [%] | 94.9 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 20 * | lithium sulfate, ammonium sulfate, citrate, 2-propanol, glycerol, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | citrate | 0.1 (M) | pH5.6 |
2 | 1 | reservoir | 1.0 (M) | ||
3 | 1 | reservoir | ammonium sulfate | 0.75 (M) | |
4 | 1 | reservoir | 2-propanol | 2 (%) | |
5 | 1 | reservoir | glycerol | 30 (%(v/v)) | |
6 | 1 | drop | protein | 10 (mg/ml) | |
7 | 1 | drop | HEPES | 0.1 (M) | pH7.0 |
8 | 1 | drop | 0.6 (M) |