1MOK
NADPH DEPENDENT 2-KETOPROPYL COENZYME M OXIDOREDUCTASE/CARBOXYLASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-02-19 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.78 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.600, 87.500, 100.700 |
| Unit cell angles | 72.00, 73.40, 69.81 |
Refinement procedure
| Resolution | 20.000 * - 2.800 |
| R-factor | 0.238 |
| Rwork | 0.225 |
| R-free | 0.27600 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mo9 |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.098 * |
| Total number of observations | 213523 * |
| Number of reflections | 48234 * |
| Completeness [%] | 92.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 * | 294 | Jang, S.B., (2001) Acta Crystallogr., D57, 445. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | 2-KPCC | 10 (mM) | |
| 3 | 1 | reservoir | ammonium acetate | 0.17 (M) | |
| 4 | 1 | reservoir | trisodium citrate dihydrate | 0.085 (M) | pH5.6 |
| 5 | 1 | reservoir | PEG4000 | 25.5 (%(w/v)) | |
| 6 | 1 | reservoir | glycerol | 15 (%(v/v)) |
