1MML
MECHANISTIC IMPLICATIONS FROM THE STRUCTURE OF A CATALYTIC FRAGMENT OF MMLV REVERSE TRANSCRIPTASE
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Detector technology | IMAGE PLATE |
Collection date | 1993-08-28 |
Detector | FUJI |
Wavelength(s) | 0.98 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.100, 84.100, 74.000 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.197 * |
R-free | 0.25900 * |
RMSD bond length | 0.014 * |
RMSD bond angle | 0.032 * |
Data reduction software | DENZO |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 * |
High resolution limit [Å] | 1.800 * |
Rmerge | 0.038 |
Total number of observations | 63951 * |
Number of reflections | 27086 * |
Completeness [%] | 96.3 * |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 20 * | used as seeds * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 40 (mg/ml) | |
2 | 1 | drop | MES | 50 (mM) | |
3 | 1 | drop | 0.25 (M) | ||
4 | 1 | drop | dithiothreitol | 1 (mM) | |
5 | 1 | reservoir | Tris | 50 (mM) | |
6 | 1 | reservoir | 0.2 (M) | ||
7 | 1 | reservoir | PEG4000 | 20 (%) |