1MHX
Crystal Structures of the redesigned protein G variant NuG1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2001-04-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 49.458, 49.458, 103.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.300 - 1.800 |
R-factor | 0.261 |
Rwork | 0.212 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pga |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.300 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.116 * | |
Number of reflections | 6383 * | |
Completeness [%] | 95.4 | 92.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 295 | n-propanol, sodium formate, tris-hcl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 10 (mM) | pH7.5 |
2 | 1 | reservoir | n-propanol | 8 (%) | |
3 | 1 | reservoir | sodium formate | 3.6 (M) |