1M64
Crystal structure of Q363F mutant flavocytochrome c3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-04 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.520, 88.886, 91.194 |
Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.800 |
Rwork | 0.163 |
R-free | 0.22370 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qjd |
RMSD bond length | 0.012 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 * | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.062 | 0.176 |
Total number of observations | 604721 * | |
Number of reflections | 108491 | |
<I/σ(I)> | 15.8 | 4.6 |
Completeness [%] | 96.9 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 * | 4 * | TrisHCl, sodium chloride, sodium fumarate, PEG8000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.8-8.5 |
2 | 1 | reservoir | 80 (mM) | ||
3 | 1 | reservoir | PEG8000 | 16-19 (%) | |
4 | 1 | reservoir | PEG8000 | 10 (mM) | |
5 | 1 | drop | protein | 6 (mg/ml) | |
6 | 1 | drop | Tris-HCl | 10 (mM) | pH8.5 |