1M2V
Crystal Structure of the yeast Sec23/24 heterodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.309, 126.371, 180.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 * - 2.750 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25300 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Sec23 from PDB ENTRY 1M2O |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.420 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.920 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.062 | 0.252 * |
| Total number of observations | 190671 * | |
| Number of reflections | 49463 | |
| Completeness [%] | 91.2 | 63.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 22 * | 1.0 M ammonium formate, 18 mM dithiothreitol, 10 % (w/v) xylitol, 0.1 M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP at 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 35 (mg/ml) | |
| 2 | 1 | drop | xylitol | 15 (%(w/v)) | |
| 3 | 1 | drop | ammonium formate | 1.0 (M) | |
| 4 | 1 | drop | dithiothreitol | 18 (mM) | |
| 5 | 1 | drop | HEPES | 0.1 (M) | pH7.5 |
| 6 | 1 | reservoir | ammonium formate | 1.9 (M) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
