1M2O
Crystal Structure of the Sec23-Sar1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-11-05 |
| Detector | KODAK |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.196, 151.158, 271.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.850 - 2.500 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.29500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.470 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.046 | 0.237 * |
| Total number of observations | 289977 * | |
| Number of reflections | 63872 | |
| Completeness [%] | 92.9 | 79.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 22 * | 12% (w/v) PEG 1500, 10% (w/v) isopropanol, 0.2 M ammonium acetate, 50 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP at 270K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 44 (mg/ml) | |
| 2 | 1 | reservoir | PEG1500 | 12 (%(w/v)) | |
| 3 | 1 | reservoir | isopropanol | 10 (%(v/v)) | |
| 4 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 5 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
