1LYK
THE IMPACT OF THE PHYSICAL AND CHEMICAL ENVIROMENT ON THE MOLECULAR STRUCTURE OF COPRINUS CINEREUS PEROXIDASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1994-09-20 |
Detector | RIGAKU RAXIS IIC |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 77.700, 128.410, 75.480 |
Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
R-factor | 0.179 * |
Rwork | 0.179 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.560 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.085 | 0.490 |
Total number of observations | 104109 * | |
Number of reflections | 41462 | |
Completeness [%] | 83.3 | 72.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7 | 293 | 18% PEG, 0.35M MgCl2, 10 mM KCN, pH 7.0, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
2 | 1 | reservoir | 0.35 (M) | ||
3 | 1 | reservoir | 10 (mM) | ||
4 | 1 | drop | protein | 20-25 (mg/ml) |