1LTQ
CRYSTAL STRUCTURE OF T4 POLYNUCLEOTIDE KINASE
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-03-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97873 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.585, 93.684, 124.165 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.330 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.26100 |
Structure solution method | SAD |
RMSD bond length | 0.007 * |
RMSD bond angle | 1.400 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.073 | 0.288 |
Total number of observations | 191084 * | |
Number of reflections | 38288 | |
<I/σ(I)> | 12.8 | 5.7 |
Completeness [%] | 99.1 | 97.7 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 * | 298 | PEG 4000, potassium chloride, MES, Tris, ATP, DTT, EDTA, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris | 10 (mM) | pH7.4 |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | dithiothreitol | 1 (mM) | |
4 | 1 | drop | EDTA | 0.1 (mM) | |
5 | 1 | drop | ATP | 0.1 (mM) | |
6 | 1 | drop | protein | 1 (mg/ml) | |
7 | 1 | reservoir | PEG4000 | 5-10 (%) | |
8 | 1 | reservoir | MES | 0-5 (mM) | pH6.5 |