1LTL
THE DODECAMER STRUCTURE OF MCM FROM ARCHAEAL M. THERMOAUTOTROPHICUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-12-08 |
Detector | SBC-2 |
Wavelength(s) | 1.03320 |
Spacegroup name | H 3 2 |
Unit cell lengths | 192.321, 192.321, 365.539 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 * - 3.000 |
Rwork | 0.245 |
R-free | 0.29500 |
Structure solution method | SIR + SAD |
RMSD bond length | 0.021 * |
RMSD bond angle | 4.207 * |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 * | |
High resolution limit [Å] | 3.000 | |
Rmerge | 99.900 * | 0.470 * |
Total number of observations | 835966 * | |
Number of reflections | 56524 * | |
Completeness [%] | 100 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 18 * | Hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
2 | 1 | reservoir | sodium citrate | 0.8 (M) | |
3 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
4 | 1 | drop | protein | 20 (mg/ml) |