1LNI
CRYSTAL STRUCTURE ANALYSIS OF A RIBONUCLEASE FROM STREPTOMYCES AUREOFACIENS AT ATOMIC RESOLUTION (1.0 A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 1995-08-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.934 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.200, 77.800, 38.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.500 - 1.000 |
R-factor | 0.119 * |
Structure solution method | STARTING MODEL WAS USED WITHOUT MR |
Starting model (for MR) | 1rgg |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | STARTING (MODEL WAS USED WITHOUT MR) |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.500 | 1.020 |
High resolution limit [Å] | 1.000 | 1.000 |
Rmerge | 0.042 | 0.539 |
Number of reflections | 101172 * | |
<I/σ(I)> | 28.7 | 2.4 |
Completeness [%] | 97.5 | 91.1 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (%) | |
2 | 1 | reservoir | phosphate | 0.1 (M) | pH7.2 |
3 | 1 | reservoir | ammonium sulfate | 25 (%sat) | |
4 | 1 | reservoir | glycerol | 25 (%) |