1LLD

MOLECULAR BASIS OF ALLOSTERIC ACTIVATION OF BACTERIAL L-LACTATE DEHYDROGENASE

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	105.800, 131.400, 63.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.179
RMSD bond length	0.016
RMSD bond angle	0.037
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.061 *
Number of reflections	64557 *
Completeness [%]	77.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		taken from Iwata, S. et al (1989). J. Biochem., 106-558-559. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8.2 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop		150 (mM)
4	1	reservoir	PEG6000	10 (%(v/v))
5	1	reservoir	MES	50 (mM)
6	1	reservoir	NADH	2.5 (mM)
7	1	reservoir	sodium oxamate	2.5 (mM)