1LKC
Crystal Structure of L-Threonine-O-3-Phosphate Decarboxylase from Salmonella enterica
Replaces: 1KUSExperimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.94645, 0.97915, 0.97926, 1.0205 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.960, 101.550, 117.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.201 * |
| Rwork | 0.203 |
| R-free | 0.23500 * |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.690 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.069 * | 0.243 |
| Number of reflections | 37844 | |
| <I/σ(I)> | 37 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | ammonium phosphate, KCl, glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | pH7.0 |
| 2 | 1 | drop | 150 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 2 (mM) | |
| 4 | 1 | drop | protein | 10 (mM) | |
| 5 | 1 | reservoir | ammonium phosphate | 1.5 (M) | |
| 6 | 1 | reservoir | 20 (mM) | ||
| 7 | 1 | reservoir | glycerol | 5 (%) | |
| 8 | 1 | reservoir | Bis-Tris propane | 50 (mM) | pH6.5 |
