1LHN
CRYSTAL STRUCTURE OF THE N-TERMINAL LG-DOMAIN OF SHBG IN COMPLEX WITH 5ALPHA-ANDROSTANE-3BETA,17ALPHA-DIOL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8453 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.900, 103.900, 84.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| Rwork | 0.198 |
| R-free | 0.23900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2s |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.028 * | 0.125 * |
| Total number of observations | 105549 * | |
| Number of reflections | 11516 | |
| Completeness [%] | 96.1 * | 94.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 * | Grishkovskaya, I., (1999) Acta Crystallogr., D55, 2053. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | imidazole-HCl | 80 (mM) | pH7.5 |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | DHT | 0.010 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 10 (%) | |
| 6 | 1 | reservoir | PEG4000 | 20 (%) | |
| 7 | 1 | reservoir | sodium HEPES | 100 (mM) | pH7.5 |
